Fig. 3: In vitro temperature entrainment of the Kai-protein oscillators.
From: Evolutionary origins of self-sustained Kai protein circadian oscillators in cyanobacteria
a Fraction of phosphorylated KaiC (P-KaiC) of the AγBγCγ set at pH 7.0. b P-KaiC of the ASeBSeCSe set at pH 8.0. Each set was reconstituted at 0 h (red circles), 6 h (blue circles), and 12 h (gray circles) intervals and incubated at 30 °C for 36, 30, and 24 h, respectively. After incubation, the pre-incubated samples were exposed to temperature cycles (red lines) of 9 h at 40 °C and 9 h at 30 °C (9H9L: top), 10 h at 40 °C and 10 h at 30 °C (10H10L: middle), or 12 h at 40 °C and 12 h at 30 °C (12H12L: bottom).
