Fig. 7: Combining CNM and CMT mutations shows long-term improvement on muscle and nerve structure.

A–C Muscle mass of (A) TA (from left to right ^**P = 0.0013, ^**P = 0.0053, ^*P = 0.0138), (B) Gastrocnemius (from left to right ^***P = 0.0005, ^*P = 0.0137, ^*P = 0.0271), and (C) Soleus (from left to right ^**P = 0.0073, ^**P = 0.0021, ^**P = 0.0020), normalized to body weight at 1 year (5 ≤ n ≤ 15). D TA fibers distribution based on their MinFeret diameter (n = 6). E Proportion of small fibers (MinFeret < 40 µm) in TA sections (n = 6, from left to right ^**P = 0.0097, ^**P = 0.0025, ^****P < 0.0001). D Each n represents a mouse, and the graph shows mean ± SD. A–C, E Each dot represents a mouse. Values are represented as mean ± SD. F TA transversal sections stained with hematoxylin-eosin (HE, upper panel, n = 6 biologically independent samples per group). Internalized nuclei are indicated by black arrows. Scale = 50 µm. G Electron microscopy images of sciatic nerve transversal sections (lower panel, n = 3 biologically independent samples per group). Scale bar = 10 µm. H–J Violin plots showing (H) g-ratio (=\(\frac{{axon\; diameter}}{{axon}+{myelin\; diameter}}\)), (I) axon diameter, and (J) myelin thickness of sciatic nerve myelinated fibers (804 ≤ n = nerves ≤ 2006, n = mice = 3, ^****P < 0.0001). H–J Each fiber is plotted. A, B, E Two-sided ANOVA test. C, H–J Two-sided Kruskal–Wallis test. Source data are provided as a Source Data file.