Fig. 3: Centriolar SSNA-1 localizes between the microtubule-containing outer wall and the cartwheel.

A–C Ultrastructure Expansion Microscopy (U-ExM) of centrioles from gonad spreads. The schematic shown to the right of each set of images depicts the orientation and configuration of centrioles. A Co-localization of SSNA-1, HA::SAS-4, and α/β-tubulin from two distinct centrioles showing a top view (centriole #1) and side view (centriole #2). SSNA-1 is localized inside the microtubule outer wall. Arrowhead indicates emergence of a daughter centriole that stains weakly for HA::SAS-4 but not SSNA-1. Scale bars = 100 nm. B Co-localization of SSNA-1, SAS-6::HA, and α/β-tubulin. SSNA-1 is localized outside of the cartwheel defined by SAS-6::HA. Arrowhead indicates emergence of a daughter centriole that stains positive for SAS-6::HA but not SSNA-1. Scale bar = 100 nm. C Co-localization of SSNA-1 and HA::ZYG-1. Scale bar = 200 nm. D The measured diameters of each protein are plotted with each point representing a measurement from a single centriole. The mean and standard deviation are shown. n = 28 (SAS-6::HA), 32 (SSNA-1), 55 (α/β-Tubulin), 27 (HA::SAS-4), and 13 (HA::ZYG-1). E Diagram indicating the position of each protein within the centriole. SSNA-1 localizes between SAS-6::HA and α/β-tubulin. F Co-localization of SSNA-1 and SAS-1::3 × Flag in centrioles from gonad spreads. Scale bar = 100 nm. To the right is a signal intensity profile plot of SSNA-1 and SAS-1::3 × Flag. G Co-localization of SSNA-1 and SAS-1::3 × Flag in embryonic centrioles. Scare bar = 100 nm. Source data are provided as a Source Data file.