Fig. 6: Extra centrioles arise early during the cell cycle in ssna-1(Δ) embryos. | Nature Communications

Fig. 6: Extra centrioles arise early during the cell cycle in ssna-1(Δ) embryos.

From: C. elegans SSNA-1 is required for the structural integrity of centrioles and bipolar spindle assembly

Fig. 6

Time lapse images from wild-type or ssna-1(Δ) embryos expressing mCherry::histone and GFP::SAS-7. The first and last frames are shown at the top while enlargements show intervening time points with only one spindle pole shown beginning in anaphase (time point 3:00). In wild-type embryos two GFP::SAS-7 foci (corresponding to a disengaged centriole pair) first become visible at each spindle pole during telophase (t = 9:00, yellow and magenta arrowheads). During the ensuing cell cycle, this cell assembles a bipolar spindle (t = 18:00). In ssna-1(Δ) embryos, two GFP::SAS-7 foci first become apparent during prometaphase (t = 1.0, yellow and magenta arrowheads). During telophase when centriole separation normally occurs, a third GFP::SAS-7 spot appears (t = 8.0, cyan arrowhead). During the ensuring cell cycle this cell assembles a tripolar spindle (t = 17:00). Bar = 10 μm.

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