Fig. 2: Characterization and application of xylose-responsive promoters. | Nature Communications

Fig. 2: Characterization and application of xylose-responsive promoters.

From: Modular deregulation of central carbon metabolism for efficient xylose utilization in Saccharomyces cerevisiae

Fig. 2

a Characterization of a set of xylose-responsive promoters on both glucose and xylose. The constitutive promoter pTEF1 was used as an internal standard. The green fluorescent protein (GFP) was used as a reporter. b Growth curves of three engineered xylose metabolizing strains. Two xylose-responsive promoters (pADH2 and pSFC1), as well as a constitutive promoter (pTEF1), were used to control the expression of XI, an initial and key enzyme for xylose metabolism. c Evaluating the conversion efficiency of malonyl-CoA to 3-HP. Two constitutive promoters (pENO2 and pTEF1) and a xylose-responsive promoter (pALD4) were used to control the expression of MCRN. Promoter pHXT7 was used to control the expression of MCRCm. For (a), strains were cultivated in a BioLector using a minimal medium with 2% glucose or xylose as the carbon source. For (b), strains were cultivated in a Growth Profiler using a minimal medium with 2% xylose as the carbon source. For (c), strains were cultivated in shake flasks using a minimal medium with 2% xylose as the carbon source. All data represent the mean derived from n = 3 biologically independent samples, with error bars indicating the standard deviation. Source data are provided as a Source Data file.

Back to article page