Fig. 1: WNK1 is an essential dependency in AML. | Nature Communications

Fig. 1: WNK1 is an essential dependency in AML.

From: WNK1 signalling regulates amino acid transport and mTORC1 activity to sustain acute myeloid leukaemia growth

Fig. 1

a Immunoblot of WNK1 in Cas9-expressing MA9 leukaemia cells expressing either a non-targeting (NCtrl) sgRNA or two different sgRNAs targeting Wnk1. Ponceau S staining was used as a loading control. Blots are from one representative experiment (n = 1). b, c Drop-out growth competition assays showing the relative percentage over time in days (D) of the indicated sgRNA-positive (GFP positive) Cas9-expressing MA9 leukaemia cells (b), mouse embryonic fibroblasts (MEFs) (c). An sgRNA against an essential gene (Rps19) was used as a positive control and a non-targeting sgRNA (NCtrl) was used as a negative control. Data shown are from one representative experiment (n = 1). d Drop-out growth competition assays showing the relative percentage over time in days of the indicated Cas9-expressing human AML cells that express a WNK1 sgRNA. An sgRNA against an essential gene (RPS19) was used as a positive control and a non-targeting sgRNA (NCtrl) was used as a negative control. Data shown for NOMO-1, HL-60, U937, and MOLM-13 are from one representative experiment (n = 1). Data shown for THP-1 and MV4-11 are from one of two independent experiments (n = 2). The driving oncogene is indicated for each of the AML cell lines.

Back to article page