Fig. 1: Association of GSK3 and Axin with LRP6.
a Top, cartoon of LRP6-GFP, with transmembrane domain (TM) and wt (PPPSPxS) or m10 mutant (PPPAPxA) motifs A-E as gray bars; brackets, L1LRP6 and L2LRP6 fragments used for NMR; below, cartoons of GSK3β-HA and FLAG-Axin1, with folded domains (RGS, DIX) or α-helices (GIR, GSK3-interacting region; BIR, β-catenin-interacting region) as gray boxes; positions of mutations blocking ligand binding (VE > GR, L396Q) or catalytic activity (K85R) are indicated below cartoons; black, tags used for coIP assays. b CoIP assays in transiently transfected HEK293T cells; shown are Western blots probed with antibodies (α) as indicated on the right following immunoprecipitation (IP); numbers underneath blots indicate mean values relative to wt (= 1.00) as obtained by densitometry of IP blots normalized to corresponding input blots, each showing similar results across three independent experiments; left, positions of molecular weight markers (in kDa).
