Fig. 3: Binding affinities between LRP6 and Axin or GSK. | Nature Communications

Fig. 3: Binding affinities between LRP6 and Axin or GSK.

From: Wnt signalosome assembly is governed by conformational flexibility of Axin and by the AP2 clathrin adaptor

Fig. 3

ITC profiles of 50 μM (a) Lip-A5Axin, (b) GSK3β, (c) Lip-DED4 > A or (d) Lip-RKR > A titrated with 500 μM Lip-L1LRP6 (see also Fig. 2); in each panel, the KD value was calculated from three independent experiments, and its standard deviation is given. The non-sigmoidal curve and large standard error of fit in the case of GSK3β (b) indicates that its binding to Lip-L1LRP6 is barely detectable. Note also that the molar ratios are <1 in the case of Lip-A5Axin because of the partial degradation during the preparations of wt and mutant Lip-A5Axin proteins (Supplementary Fig. 3), suggesting that the calculated KD values are underestimates.

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