Fig. 1: Single-cell landscape of T-cell responses in acute HBV patients. | Nature Communications

Fig. 1: Single-cell landscape of T-cell responses in acute HBV patients.

From: Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice

Fig. 1

a A schematic workflow of the study design (top) and the clinical time course of five acute HBV infection (AHB) patients (bottom) (created with BioRender.com). b Uniform manifold approximation and projection (UMAP) visualization of PBMCs and liver cells, colored by cluster. CD4 TEF GZMK: Granzyme K+ effector CD4+ T cells; CD8 TEX GZMA: Granzyme A+ exhausted CD8+ T cells. c Box plots showing the fold enrichment of cell type proportions between AHB patients (n = 5) and healthy donors (HDs, n = 5). In each plot, the box represents the interquartile range, with the median indicated by a horizontal line. The lower whisker extends to the minimum (lowest value within 1.5×IQR) and the upper whisker extends to the maximum (highest value within 1.5 × IQR). d Pathway enrichment analysis of T cells in AHB patients versus HDs. e Box plots illustrating the cytotoxicity scores of TEF and TEX CD8+ T cell subsets in AHB patients (n = 5) versus HDs (n = 5). Each box plot displays the minimum, lower quartile, median, upper quartile, and maximum values. f Clonal status percentages of T cells across AHB patients and HDs. g Proportion of single and expanded clones (left) and quantification of clonal expansion (right) in T cell clusters in AHB patients (n = 5) versus HDs (n = 5). The point estimates (odds ratios) are depicted with error bars representing the 95% confidence intervals. h Gene set enrichment analysis (GSEA) of myeloid cell clusters in AHB patients versus HDs. ISG: Interferon-stimulated genes. i GSEA of dendritic cells (DCs) in AHB patients versus HDs. j Circos plot illustrating the prioritized interactions mediated by ligand-receptor pairs between hepatic-enriched myeloid cell subsets and activated and cycling CD8+ T cells within the liver. Statistical analysis was performed using two-tailed Mann-Whitney tests (c), one-tailed Fisher’s exact test (d), two-tailed Student’s t-tests (e), two-tailed Fisher’s exact test (g) and Kolmogorov-Smirnov (K-S) test followed by Benjamini-Hochberg procedure (h, i). Statistically significant results are labeled (*p < 0.05, **p < 0.01, ***p < 0.001).

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