Fig. 2: Intrahepatic Batf3+XCR1+CCR7- cDC1s drive CD8+ T cell response against HBsAg. | Nature Communications

Fig. 2: Intrahepatic Batf3+XCR1+CCR7- cDC1s drive CD8+ T cell response against HBsAg.

From: Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice

Fig. 2

a UMAP plot of 4829 single liver cells as illustrated in Supplementary Fig. 3a. b Dot plot of selected canonical cell markers across populations detailed in (a). c Representative FACS plots and percentages of H-2Kb-SIINFEKL-expressing antigen-presenting cells (APCs) in WT mouse livers at days 0 and 2.5 post-pHBV1.3/OVA257-264 hydrodynamic injection (HDI) (n = 3). d Representative Violet tracer dilution and proliferation index of OT-I T cells after a 3-day culture alone or with liver-sorted APCs from WT mice post-1 day pHBV1.3/OVA257-264 HDI (n = 6 biologically independent replicates). e, f Violet tracer-labeled OT-I T cells were transferred into WT or Batf3 KO mice, followed by pHBV1.3/OVA257-264 HDI for 3.5 days. e Representative Violet tracer dilution and proliferation index (n = 6). f Representative FACS plots and percentages of IFN-γ+ or GzmB+ OT-I T cells (n = 6). g, h Violet tracer-labeled OT-I T cells were transferred into WT recipients treated with anti-XCL1 versus control antibody, followed by pHBV1.3/OVA257-264 HDI for 3.5 days. g Representative Violet tracer dilution and proliferation index (n = 6). h Representative FACS plots and percentages of IFN-γ+ or GzmB+ OT-I T cells (n = 6). i, j Same as (g, h) but using HBV Env28 T cells as donors and BALB/C mice as recipients receiving pHBV1.3 for HDI (n = 3). k, l Representative FACS plots and percentages of HBV ENV353 tetramer-specific CD8+ T cells in Batf3 KO versus WT mice at day 5 post-pHBV1.3 HDI (n = 6), and representative FACS plots and percentages of EdU+ cells among these tetramer-specific CD8+ T cells (n = 3). m Intrahepatic HBsAg levels in WT and Batf3 KO mice on day 14 post-pHBV1.3 HDI (n = 15 fields from 5 mice). Scale bars, 50 μm. At least two independent experiments were performed. The horizontal lines represent median values. Statistical significance was calculated using one-way ANOVA with Dunnett’s multiple comparisons test (d) and two-tailed Student’s t-tests (c, e-m). Significance levels are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data and exact p-values are provided in the Source Data file.

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