Fig. 5: B cells acquire nonopsonized HBsAg in situ.

a Schematic illustrating the targeting strategy for generating HBsAg-specific Ig knock-in mice, named S129G1_HL. b Representative FACS plots and percentages of HuCκ⁺, HBsAg-binding cells in the blood from S129G1_HL versus WT mice, gated on B220⁺ cells (n = 3). c Schematic showing the time course of the B cell response to HBsAg. d Representative histograms and geoMFI data of Nur77 expression in S129G1_HL B cells in the spleens from WT recipients treated with both anti-LFA-1 and anti-α4 versus control antibodies, at 21 hours post-pHBV1.3 HDI (n = 3). e Representative histograms and geoMFI data of Nur77 expression in S129G1_HL B cells in the dLNs from WT recipients treated with anti-CD62L versus control antibodies, at 21 hours post-pHBV1.3 HDI (n = 3). f Representative histograms and geoMFI data of Nur77 expression in the livers from mice with both splenectomy and FTY720 versus control treatments, at 21 hours post-pHBV1.3 HDI (n = 3). g Immunohistochemistry of spleens and dLNs from WT recipients at 18 hours post-pHBV1.3/OVA323-339 HDI, showing S129G1_HL B cells (HuCκ, blue) and endogenous B cells (IgD, brown). Scale bars, 50 μm. h-k Representative histograms and geoMFI data of Nur77 expression in transferred S129G1_HL B cells in the spleens, dLNs, and livers from C3 KO (h, n = 3), FcγRIIB KO (i, n = 3), Zbtb46-DTR chimeras treated with DT ( j, n = 3), and WT mice treated with CLD liposomes (k, n = 3), compared to their respective controls, at 21 hours post-pHBV1.3 HDI. At least two independent experiments were performed. The horizontal lines represent median values. Statistical significance was calculated using two-tailed Student’s t-tests. Significance levels are indicated as ****p < 0.0001. Source data and exact p-values are available in the Source Data file.