Fig. 6: B cells initiate CD4+ T cell responses sequentially in follicular and interfollicular areas. | Nature Communications

Fig. 6: B cells initiate CD4+ T cell responses sequentially in follicular and interfollicular areas.

From: Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice

Fig. 6

a Immunofluorescence of S129G1HL B cells (HuCκ, green), OT-II T cells (CD45.1, red), and endogenous B cells (B220, blue) in the spleens and dLNs of WT recipients at the indicated time points post-pHBV1.3/OVA323-339 HDI. The boxed area indicates regions shown at higher magnification. Scale bars, 50 μm. b Schematic illustration of the spatial organization of B and T cell zones and their interfaces in spleen and dLN (top), and the relative proportions of S129G1HL B cells (bottom, left) and OT-II T cells (bottom, right) localized in each anatomical region at the indicated time points. (middle: n = 6 white pulp areas from 3 mice; bottom: n = 6 LN slices from 6 mice). The data represent the relative proportions of each group. Three independent experiments were performed. Source data are available in the Source Data file.

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