Fig. 6: SPP1 + TAM and tumor invasiveness in PitNETs. | Nature Communications

Fig. 6: SPP1 + TAM and tumor invasiveness in PitNETs.

From: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

Fig. 6

a Prevalence of each immune stroma cluster, estimated by Ro/e score. b−d Proportions of patients with different invasion (b), relapse (c) and size (d) statuses comparing TAM_SPP1-enriched (n = 5) versus TAM_SPP1-depleted (n = 25) groups. P-values were determined by two-sided chi-square test. e Patient proportions with varying invasion statuses, using bulk sequencing data from Zhang 202251 (n = 80 (SPP1 high) and 114 (SPP1 low)) and Zhang 202450 (n = 16 (SPP1 high) and 16 (SPP1 low)). p-values calculated by two-sided chi-square test. f Bar plot showing the proportion of SPP1+ TAMs in invasive (n = 14) and non-invasive (n = 33) PitNETs, as determined by multiplex immunofluorescence staining data. p-value was calculated using the two-sided wilcoxon signed-rank test. Boxes span the interquartile range (IQR) and whiskers extend to points that lie within 1.5 IQRs of the lower and upper quartile. Center line is drawn at the median. g Bar plot of top 5 ligand-receptor pairs with the highest interaction probabilities between invasive and noninvasive PitNETs. h Chord diagrams illustrating interactions between TAM subtypes and tumor clusters via SPP1-ITGAV/ITGB1 ligand-receptor pair, with line width indicating interaction intensity. i Signature scores of MAPK pathways (top) and PI3K-AKT pathways (bottom) for invasive (n = 44,607 cells) and non-invasive (n = 103,032 cells) tumor clusters. p-values were determined using the two-sided wilcoxon signed-rank test. Boxes span the interquartile range (IQR) and whiskers extend to points that lie within 1.5 IQRs of the lower and upper quartile. Center line is drawn at the median. j Spatial gene expression patterns of SPP1, ITGAV, and ITGB1 in samples SCA5 (invasive tumor) and SCA1 (non-invasive tumor). k Representative multiplex immunofluorescence staining of human PitNET tissue. DAPI (blue), CD68 (yellow), SPP1 (orange), ITGB1 (red), ITGAV (green), and tumor marker TPIT (magenta) are shown in individual and merged channels (n = 3 biological replicates). Scela bar, 100 µm. Source data are provided as a Source Data file for (a−i).

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