Fig. 8: ITGB1 knockdown inhibits osteopontin (OPN)-induced proliferation and invasion in pituitary tumor cells. | Nature Communications

Fig. 8: ITGB1 knockdown inhibits osteopontin (OPN)-induced proliferation and invasion in pituitary tumor cells.

From: Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression

Fig. 8

a Western blot analysis of ITGB1 expression in GH3, AtT-20, and TtT/GF cell lines. b Semi-quantitative analysis of ITGB1 in GH3, AtT-20, and TtT/GF cell lines (n = 3 biological replicates). c CCK-8 cell viability assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments (n = 3 biological replicates). d Transwell invasion assay of GH3, AtT-20, and TtT/GF cell lines under specified treatments (n = 3 biological replicates). e Quantitative analysis of Transwell invasion assay in GH3, AtT-20, and TtT/GF cell lines under specified treatments (n = 3 biological replicates). Scale bar, 100 µm. Two-sided unpaired t-test was performed for (b−e). Data are presented as mean values +/- SD for (b−e). Source data are provided as a Source Data file for (a−e).

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