Fig. 7: Mitochondria and mtDNA analysis of tissues from 24-month-old mice.
From: Modelling POLG mutations in mice unravels a critical role of POLγΒ in regulating phenotypic severity
a Long-range PCR performed in DNA isolated from brain, liver, and SKM of 24-month-old Polg+/+ (n = 3) and Polg+/Y933C (n = 5) mice. Primers amplified a fragment of 15,781 bp of the mtDNA. The bands were visualized by SYBR™ safe staining. Each lane represents a biological replicate. Both females and males were used. b Real-Time qPCR quantification of mtDNA content in liver, brain, and SKM of 24-month-old Polg+/+ (n = 3) and Polg+/Y933C (n = 5) mice. Data are presented as mean ± S.E.M. Both females and males were used. c Representative EM images from Polg+/+ and Polg+/Y933C SKM. The size bar represents 500 nm. d Quantification of the mitochondrial area, length and width (n = 80 mitochondria/genotype, n = 15 independent TEM images per condition from 2 independent animals). Error bars indicate ±S.E.M. p values were calculated by two tailed unpaired Student’s t test. e Quantification of mitochondria with aberrant morphologies in SKM. n = 15 independent TEM images per condition from 2 independent animals. Two-tailed χ2 test was used for statistical analysis. Source data are provided as a Source Data file.
