Fig. 7: Requirement for bubble formation 3’ of nic.

a Requirement for a DNA bubble 3’ of nic. Five 2D-classes are shown for five different relaxosome complexes reconstituted using five different types of DNAs. These DNAs are as described in Fig. 1c and Supplementary Fig. 2. Two types of classes are observed, regrouped into 2 rows. The first row reports on 2D-classes where the TE region is fuzzy, i.e. in those complexes, the TE domain is not bound to its binding site. The second row reports on 2D-classes where the TE region is well defined i.e. in those complexes, the TE domain is engaged with its binding site. See main text for description and interpretation of results. b Requirement for a DNA bubble 3’ of nic (continued). Same as panel a but reporting the corresponding 3D maps. The top row shows the 3D maps where the TE region is disordered: A map of similar resolution from initial processing of ds_{-27_+143}-R dataset is shown for comparison. Bottom row shows the maps where TE is ordered, indicating its binding to the ssDNA. A map of similar resolution of ss_{-27_+8}ds_{+9_+143}-R is shown for comparison. Regions of the maps corresponding to the TE domain is coloured orange. See Methods section and Supplementary Table 2 for details. Inset shows the relaxosome structure in ribbon in the same orientation as the maps and with the TE domain coloured in orange.