Fig. 3: FOXA2 promotes SCLC metastasis.
From: FOXA2 promotes metastatic competence in small cell lung cancer
a Clinical validation using the TMA tissue sections. FOXA2 H-score of a TMA of limited stage SCLC patients comparing those with no relapse (N = 11) vs. relapse (N = 15) (* < 0.05, P = 0.049, two-sided Student’s t-test). b Relapse-free survival of patients with FOXA2low and FOXA2high tumors (** < 0.01, P = 0.0033, log-rank test). c–f FOXA2 KD with short-harpin RNA (sh) in cell lines H1836 (c, d) and SHP-77 (e, f) and metastatic burden assessment. Total photon reflux [p/s] is indicated in graphs (c, e), and the number of macroscopic metastases after euthanasia are indicated in graphs (d, f). Mean ± SEM (* < 0.05, ** <0.01, *** <0.001, **** <0.0001, one-way ANOVA. c; P = 6.0 × 10-4(top), P = 7.0 × 10-4(bottom), d P = 4.6 × 10-4(left), P = 1.0 × 10-3(right), e P = 0.033(top), P = 2.6 × 10-4(bottom), f P = 0.014(left), P = 9.6 × 10-3(right)). g, h Mouse Foxa2 overexpression (OE) in KD cell line SHP-77. Total photon reflux [p/s] is indicated in graph (g) and the number of microscopic liver metastases in each liver lobe after euthanasia are indicated in graph (h). Mean ± SEM (** < 0.01, g; P = 2.3 × 10-3, h; P = 3.6 × 10-3, two-sided Student’s t-test). i FOXA2 immunohistochemistry (IHC) of H1963 subcutaneous tumor and its ovarian metastasis generated by intra-cardiac injection. Scale bar: 500 μm and 100 μm (inset). j, k Schematic of the generation of ovarian metastasis (Ovamet) subline (j) and FOXA2 western blot of parental and ovarian metastasis subline (k). Western blot result is a representative data of one of the 3 individual experiments. Created in BioRender. Rudin C. (2025) https://BioRender.com/58ppd5o.
