Fig. 3: Engineered SmOPT U7 variants can improve editing across multiple endogenous targets. | Nature Communications

Fig. 3: Engineered SmOPT U7 variants can improve editing across multiple endogenous targets.

From: An engineered U7 small nuclear RNA scaffold greatly increases ADAR-mediated programmable RNA base editing

Fig. 3

The original SmOPT mU7 hairpin sequence (white) or three combination variants (SmOPT-11A + mU7-3GG, SmOPT-11A + mU7-12C, and SmOPT-11A + mU7-3GG + mU7-12C) were cloned onto six antisense guide RNAs, with or without a 5′ double hnRNP A1 binding motif, and individually transfected into Landing Pad 293T cells. Following puromycin selection for successfully integrated single-copy guide RNA constructs, RNA was measured 13 days post transfection. Negative controls consist of samples where the transfected plasmid targeted a different gene. Mean values are shown for 2 biological replicates. Results across all antisense guide RNAs (n = 12; 6 gene targets +/− hnRNP) were analyzed in aggregate by two-way ANOVA; P values comparing each combination variant to the original SmOPT mU7 hairpin were calculated using Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.

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