Fig. 10: Loss of non-hematopoietic Btk decreases transcytosis of CXCL1.
From: Alveolar epithelial and vascular CXCR2 mediates transcytosis of CXCL1 in inflamed lungs
WT and Btk−/− mice were leathally irradiated and bone marrow reconstitution was performed by i.v. injection of isolated WT or Btk−/− bone marrow cells. 6 weeks after irradiation, bone marrow chimeric mice (WT donor -> WT recipient and WT donor -> Btk KO recipient) mice were intratracheally administered with sterile saline or with viable K. pneumoniae. Neutrophil recruitment into the BAL (A) and lung (B) as well as CFUs in the BAL (C) and lung (D) were assessed 24 h after infection. E Graphical abstract visualizing the main findings of this study. Data are mean ± SD, n = 3 biologically independent mice pre group, age 8–16 weeks, random gender distribution, one-way ANOVA followed by Bonferroni correction, *p < 0.05, **p < 0.01, ***p < 0.001.
