Fig. 4: Neutrophil recruitment into the alveoli is diminished in CXCR2 deficient mice upon CXCL1 but not fMLP treatment. | Nature Communications

Fig. 4: Neutrophil recruitment into the alveoli is diminished in CXCR2 deficient mice upon CXCL1 but not fMLP treatment.

From: Alveolar epithelial and vascular CXCR2 mediates transcytosis of CXCL1 in inflamed lungs

Fig. 4

Intravital microscopy of the lung was performed in CXCR2 KO mice (red = CXCR2−/−, green = CXCR2fl/flCdh5Cre+, blue = CXCR2fl/flShhCre+) and appropriate WT/Cre- controls (gray) 4 h after intratracheal injection of CXCL1 or fMLP. Neutrophils were visualized in the alveoli by intravenous administration of an AF488-coupled Gr-1 antibody before starting microscopy (A, F, K). Neutrophil numbers were determined per field of view after CXCL1 (B, G, L) or fMLP (C, H, M) treatment. BALF was collected, and neutrophil infiltration into the alveoli was assessed by flow cytometry after CXCL1 (D, I, N) or fMLP (E, J, O) stimulation. Data are mean ± SD, scale bars equal 20 µm, n = 3–4 biologically independent mice pre group, age 8–16 weeks, equal gender distribution, t-test, *p < 0.05, **p < 0.01.

Back to article page