Fig. 8: Inhibition of clathrin-mediated transcytosis reduces CXCR2-mediated transcytosis of CXCL1.
From: Alveolar epithelial and vascular CXCR2 mediates transcytosis of CXCL1 in inflamed lungs
A Primary murine lung microvascular endothelial cells (MLMVEC) and alveolar epithelial cells (AEC) were cultured on different sides of a Transwell™ insert. Transmigration of WT bone marrow-derived neutrophils towards a CXCL1 gradient was analyzed under control conditions (solvent DMSO) and after incubation of the epithelium and endothelium with the inhibitor Pitstop-2 (10 µM), MBCD (1 µM), and Dyngo 4a (30 µM) for 30 min. B CXCL1 concentrations in the upper well after 30 min (data are mean ± SD, n = 6 biologically independent samples), one-way ANOVA followed by Bonferroni correction, *p < 0.05.
