Fig. 5: Whole genome sequencing of SARS-CoV-2, BoDV-1, HAV, PV and ratHEV.
From: varVAMP: degenerate primer design for tiled full genome sequencing and qPCR
Representative coverage plots (left) and % genome recovery (right) of the different a SARS-CoV-2, b BoDV-1, c HAV, d PV and e ratHEV samples subjected to their respective tiled amplicon whole genome sequencing workflows. Coverage plots were created with BAMdash. The dotted lines indicate the mean coverages. The reference genomes used for mapping are indicated in the header of the coverage plots (individual coverage plots are given in Supplementary Fig. 2). Genome recovery was calculated as the precentage of reference nucleotides covered at least 20 fold (sp single plex, mp multiplex). Dark gray bars—ONT generated data, light gray bars—Illumina generated data.
