Fig. 5: TEAD1 regulates the key human β cell gene expression.
From: FXYD2 marks and regulates maturity of β cells via ion channel-mediated signal transduction
a Representative IB analysis of indicated phosphorylated and total proteins in EndoC-βH1 cells under the indicated conditions. b qPCR analyses of indicated gene expression in EndoC-βH1 cells. DOX-inducible TEAD1 overexpression (dTEAD1OE, DOX +) and knockdown (dTEADKD, DOX +) regulate the expression of key β cell genes, including MAFA, UCN3, and IAPP. n = 3. Unpaired two-tailed t-tests. c, Human c-peptide secretion assay (fold change to DOX (–) G3 stimulation) from dTEAD1KD EndoC-βH1 cells. n = 12. Unpaired two-tailed t-tests. d–g TEAD1 ChIP-seq analyses. The distribution of relative gene positions at the peak summit (d), enriched motif analyses (e), KEGG pathway analyses (f) in control and cFXYD2OE EndoC-βH1 cells. g Browser tracks showing TEAD1 ChIP-seq peaks at MAFA, PDX-1, NKX6-1, UCN3, IAPP, NEUROD1 loci in control and cFXYD2OE EndoC-βH1 cells. Error bars indicate SEM.
