Fig. 1: Low-risk MDS27-hiPSC lines are able to differentiation into hematopoietic progenitors in semi-solid medium and into erythroid cells in the liquid culture.

A Genotyping screening for MDS27 patient sample taken at the time of diagnoses and after disease progression as well as for iPSC clones generated from the diagnosis MDS27 patient sample. Artwork generated with powerpoint Bundle-Biology. B Bar graph showing the total number of HPCs, percentage of early hematopoietic population (CD43⁺) and late hematopoietic population (CD34⁺ CD45⁺) on day 14 of differentiation. Statistical results are presented as mean ± SEM. ns = no significant, one-way ANOVA with Dunnett’s multiple comparisons. N = 4 independent experiments. Source data are provided as a Source Data file. C Total number of CFUs from 10⁴ iPSC-HPC cells grown for 14 days in semi solid medium. Statistical results are presented as mean ± SEM. * p < 0.05, ** p < 0.001. Two-tailed unpaired t-test. N = 4 independent experiments. Exact p-values can be found in the Supplementary Data Table 5. D Number of each type of CFUs after 14 days in semisolid medium. Mean and SEM of different lines are shown. N = 4 independent experiments. Source data are provided as a Source Data file. E Relative percentage of each type of CFUs for 1 × 10⁴ of HPCs after 14 days in semisolid media. Statistical results are presented as mean ± SEM and ****p < 0.0001, **p < 0.001, *p < 0.05, ns: no significant. Two-way ANOVA with Dunnett’s multiple comparisons. N = 4 independent experiments. Exact p-values can be found in the Supplementary Data Table 5. Source data are provided as a Source Data file. F Schematic representation of the experimental set up for the erythroid differentiation of HSPCs. Artwork generated with powerpoint Bundle-Biology. G Flow cytometry contour plots for CD71 and CD235a markers on Day 0, 4, 7, 11, 14 and 18 during erythroid differentiation. N = 4 independent experiments. Source data are provided as a Source Data file. H Percentage of erythrocyte (CD71⁺), erythroblasts (CD71⁺ CD235a⁺) and mature erythrocytes (CD235a+) during erythroid differentiation. Mean and SEM are shown (ns, no significant), two-way ANOVA with Dunnett’s multiple comparisons N = 4 independent experiments. Source data are provided as a Source Data file. I Diff-quick stained cytospins showing common aberrant morphology (black arrow) observe in MDS27-C22. The pictures were taken with a Leica DM6000 at ×100 magnification. N = 3 independent experiments. J Percentage of erythroid cells with aberrant morphology. Statistical results are presented as mean ± SEM. ***p < 0.0001 and *p < 0.05. Two-way ANOVA with Dunnett’s multiple comparisons. N = 4 independent experiments. Exact p-values can be found in the Supplementary Data Table 5. Source data are provided as a Source Data file. K May–Grunwald Giemsa staining stained bone marrow smears from MDS27 patient at the time of diagnosis showing aberrant erythroid cells. The pictures were taken with a Leica DM6000 at ×100 magnification. Taken from Supplementary Fig. 1A.