Fig. 3: GFAP-positive hNSPCs express type B cell markers.
From: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling
a UMAP plot of merged scRNAseq datasets of hNSPCs in media control, hEC conditioned media, and hEC co-cultures. b Featureplots demonstrate PECAM1 (CD31) expression by hEC clusters, MKI67 expression by proliferating endothelial cells and proliferating progenitors, and GFAP and SOX2 expression by hNSPC clusters. High GFAP and SOX2 co-expression was observed in a subset of neural stem cells (red circles). c UMAP plot of hNSPC subsets from merged datasets revealed two additional clusters, neural stem cells (Type B) and early neuron progenitors. d Dot plot demonstrating the expression of type B cell markers, neural stem cell markers, proliferation markers, astrocyte markers, and neuronal markers. The average gene expression of cells in the cluster compared to the average expression across all clusters is noted by the color. The size of the dot represents the percentage of cells in the cluster expressing a particular gene. e Heatmap of the top 10 differentially expressed genes in each hNSPC cluster depicted in (c) based on average log2 fold change. Yellow represents high expression and purple is low expression.
