Fig. 9: Co-expression of GFAP and PROM1 in hNSPCs in vitro and in the human SVZ.
From: Human endothelial cells promote a human neural stem cell type B phenotype via Notch signaling
a SC23 (top row) and SC27 (bottom row) hNSPCs co-cultured for 5 days with hECs (left panels) or alone (right panels) and immunostained for GFAP and PROM1. GFAP +, PROM1+ cells are evident in co-cultures. Nuclei stained with Hoechst and scale bars 100 μm. b 2-year-old human SVZ was stained for GFAP, PROM1, CD31, and DAPI and imaged with confocal microscopy (n = 1, biological repeats at other stages in Supplementary Figs. 20, 21). Positive staining of the SVZ is evident in 20× images (scale bar, 100 μm). Higher magnification 60× images show the spatial distribution of GFAP and PROM1 and box denotes enlarged region shown in middle panels (scale bar 30 μm). Arrows in middle panels mark cells or clusters of cells with both GFAP and PROM1 (membrane) staining. The boxed cluster of cells is further enlarged in lower panels to show detail of GFAP and PROM1 co-staining.
