Fig. 4: Conditioned media of B. theta grown on distinct carbon sources has altered effects on the immune system. | Nature Communications

Fig. 4: Conditioned media of B. theta grown on distinct carbon sources has altered effects on the immune system.

From: Dietary carbohydrates alter immune-modulatory functionalities and DNA inversions in Bacteroides thetaiotaomicron

Fig. 4

a Experimental design, conditioned media from carbon sources in which B. theta grew after 24 h was sterile filtered and introduced to SPF mice splenocytes. Five days later, cell population percentages were evaluated by flow cytometry analysis and IL-10 and IL-17 cytokine concentrations in the media were measured using ELISA. b, c Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source without any bacteria growing in it) of CD8 + Ki67 + PD1+ cell percentages of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate (b) and carbon sources from PM2A plate (c). d, e Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source without any bacteria growing in it) of IL-10 concentration in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate (d) and carbon sources from PM2A plate (e). f, g Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source without any bacteria growing in it) of IL-17 concentration in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate (f) and carbon sources from PM2A plate (g). h Heatmap representing the average log2 fold change value for each of the immune parameters examined (CD8 + Ki67 + PD1+ cell percentages, IL-10 and IL-17 concentrations). Z-score was calculated for each carbon source in each parameter. Carbon sources with |Z-score| ≥ 2 in each immune parameter were marked with #. b-g Each dot represents a biological repeat 3 ≤ n ≤ 10. b, c Kruskal–Wallis ANOVA, using Dunn’s multiple comparisons test, d, f, g Ordinary one-way ANOVA, using Tukey’s multiple comparisons test, e Brown-Forsythe and Welch ANOVA, using Dunnett’s T3 multiple comparisons test. Bars represent the mean. Error bars represent s.d. *P < 0.05 and **P < 0.01, ***P < 0.001, ****P < 0.0001, all exact p-values are listed under “P-values” sheet in the Source Data file. Source data are provided as a Source Data file. Figure 3a was created in BioRender. Geva-Zatorsky, N. (2025) https://BioRender.com/82criwi.

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