Fig. 5: Boiling of B. theta conditioned media reduces some but not all conditioned media effects on the immune system.

a, b Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source, boiled or not boiled, without any bacteria growing in it) of CD8 + Ki67 + PD1+ cell percentages of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate (a) and carbon sources from PM2A plate (b). Darker colors represent not boiled media, lighter colors represent boiled media. c, d Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source, boiled or not boiled, without any bacteria growing in it) of IL-10 concentrations in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate (c) and carbon sources from PM2A plate (d). Darker colors represent not boiled media, lighter colors represent boiled media. Log2 fold change (compared to control, cells exposed to M9 minimal media and the relevant carbon source, boiled or not boiled, without any bacteria growing in it) of IL-17 concentrations in the media of splenocytes exposed to conditioned media of B. theta grown on carbon sources from PM1 plate (e) and carbon sources from PM2A plate (f). Darker colors represent not boiled media, lighter colors represent boiled media. a–f Each dot represents a biological repeat 6 ≤ n ≤ 9. Paired, tow tailed, t test for each couple of not boiled and boiled conditioned media. Bars represent the mean. Error bars represent s.d. *P < 0.05 and **P < 0.01, ***P < 0.001, ****P < 0.0001, all exact p-values are listed under “P- values” sheet in the Source Data file. Source data are provided as a Source Data file.