Fig. 2: Iron deprivation induces changes to the mitochondrial proteome, mitochondrial reactive oxygen species (mROS) production and loss of mitochondrial membrane potential.
From: Iron deficiency causes aspartate-sensitive dysfunction in CD8+ T cells
CD8+ T cells were activated as described in Fig. 1a. a mTORC1 and MYC are metabolic regulators that enable biosynthesis downstream of TCR stimulation. b, c mTORC1 activity measured via its downstream target, phospho-S6 (pS6), n = 4. Controls were treated overnight with rapamycin (rapa; 1 µM). d Heatmap of proteins in selected metabolic pathways defined using GO terms where the p value < 0.05, n = 4. Electron transport chain = GO_RESPIRATORY_ELECTRON_TRANSPORT_CHAIN, β-oxidation = GO_FATTY_ACID_BETA_OXIDATION, ribosome = GO_CYTOSOLIC_RIBOSOME, amino acid (AA) transport = GO_AMINO_ACID_IMPORT. e Mitochondrial membrane mass measured using Mitotracker green (MTG) MFI, n = 4. PCA of the protein-MS f given prior selection for proteins in the MitoCarta3.0 gene set and g of all proteins, n = 4. h, i mROS MFI measured using MitoSOX red, n = 4. j Mitochondrial membrane potential calculated as the ratio of Mitotracker deep red (MTDR) to Mitotracker green (MTG), n = 4. k SOD2 protein expression by protein-MS, n = 4. Data are mean ± SEM, where each datapoint per condition denotes cells from independent donor mice. Statistics are: b, h, k matched one-way ANOVAs with the Geisser-Greenhouse correction; e, j matched two-tailed t-test. Source data are provided as a Source Data file.
