Fig. 5: Scope of peptide substrates. | Nature Communications

Fig. 5: Scope of peptide substrates.

From: Chemoselective dual functionalization of proteins via 1,6-addition of thiols to trifunctional N-alkylpyridinium

Fig. 5

a PC8 peptide modification with compound 1. b HPLC trace of 1, the reaction mixture of PC8 and 1 (1.1 equiv), and the reaction mixture of DPS-modified PC8 peptide and compound 1 (from top to bottom). Experimental for (a, b) can be found in SI (Supplementary Fig. 64–66). Single HPLC injections were performed for qualitative analysis. c ESI-MS of 11 (calculated: 1238.9 [M]+, found: 1238.8 [M]+). d Site-selective modification of different peptides with compound 1. Reactions were performed in aqueous media (See Methods). Phosphate buffer (PB) and 4,4’-dithiodipyridine (4-DPS). Cysteine residue is in bold.

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