Fig. 5: Multicolor imaging by VIPIs. | Nature Communications

Fig. 5: Multicolor imaging by VIPIs.

From: Near-infrared II cyanine fluorophores with large stokes shift engineered by regulating respective absorption and emission

Fig. 5

A The multicolor imaging of three vials containing VIPI-1 (left, 10 μM in 10% FBS), VIPI-4 (middle, 10 μM in 10% FBS), and VIX-4 (right, 0.2 mg/mL liposomes in water) under different excitations. Blue channel: 730 nm excitation, 1000 nm LP, exposure time (ET) = 50 ms; green channel: 808 nm excitation, 1000 nm LP, ET = 30 ms; red channel: 980 nm excitation, 1150 nm LP, ET = 100 ms. B Dosing scheme for multicolor imaging experiment and representative in vivo multicolor imaging of mice (n = 3 mice). VIPI-1 (500 μM, 100 μL), VIPI-4 (500 μM, 200 μL), and VIX-4 (10 mg/mL, 200 μL) were separately administrated via gavage, clyster, and intravenous injection (i.v.). Dosing scheme (the first row); three single color channels (the second row); the merged three-color channel (the bottom). Blue channel: ET = 100 ms; green channel: ET = 100 ms; red channel: ET = 200 ms. Scale bars, 1 cm.

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