Fig. 7: Spectral properties of VIPI-4 liposomes and fluorescence imaging of mouse bones under different LP filters. | Nature Communications

Fig. 7: Spectral properties of VIPI-4 liposomes and fluorescence imaging of mouse bones under different LP filters.

From: Near-infrared II cyanine fluorophores with large stokes shift engineered by regulating respective absorption and emission

Fig. 7

The absorption (A) and normalized fluorescence (B) spectra of VIPI-4 liposomes (0.2 mg/mL) in saline. The normalized fluorescence of VIPI-4 (10 μM, dash line) was shown for reference. C The bone imaging of (C1-C3) in vivo and (C4-C6) skinned mice from ventral view after 24 h intravenous injection of VIPI-4 liposomes (20 mg/mL, 200 μL). The exposure time is shown in each image. D The SBR of images C1-C3. SBR is the fluorescence intensity ratio in the yellow solid line circle (spine area) and dashed line circle (non-bone area) with the same area (for clarity, these positions are indicated in image C3 only). The SBR values are noted on the column. E Representative images of knee joints of mice (n = 3 mice). (E1-E3) In vivo fluorescence images of knee joints of mice. (E4-E6) Fluorescence images of knee joints of mice after stripping muscle. The skeleton names are marked in images E3 and E6.

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