Fig. 4: CBP/P300 and BRD4 induce Pol II initiation and elongation, respectively, during ZGA. | Nature Communications

Fig. 4: CBP/P300 and BRD4 induce Pol II initiation and elongation, respectively, during ZGA.

From: Coordinated action of multiple active histone modifications shapes the zygotic genome activation in teleost embryos

Fig. 4

A Track view of RNA-seq and spike-in ChIP-seq after CBP/P300 and BRD4 inhibitor treatment. Data from the previous study4 are shown with *. B Heatmap showing Pol II enrichment (spike-in ChIP-seq) around gene bodies after treatment with CBP/P300 and BRD4 inhibitors. C Line plot showing the average of (B). D Schematic showing the function of BRD4 and mechanism of JQ1. E Scatter plots indicate RNA-seq expression levels in control and JQ1-treated embryos. Red and blue dots indicate significantly upregulated and downregulated genes ( | log2 fold change | > 1, adjusted p value < 0.01, RPKM > 1.5) in JQ1-treated embryos, respectively. Pink, green, and light blue dots indicate Maternal, Maternal-ZGA, and Pure ZGA genes (see Supplementary Fig. 1F–H), respectively. n = 2 biological replicates. F Venn diagram indicates overlap of ZGA genes (see Supplementary Fig. 1E), CBP/P300-dependent genes (Fig. 1J) and significantly downregulated genes in JQ1-treated embryos.

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