Fig. 5: CBP/P300-independent histone acetylation accumulates at CBP/P300-independent ZGA genes.

A Heatmap showing correlations between expression changes (RNA-seq) after CBP/P300 inhibitor treatment and histone modification enrichment levels (ChIP-seq) in normal medaka embryos. Data from the previous study⁴ are shown with *. B Track view of RNA-seq after CBP/P300 inhibitor treatment and ChIP-seq in normal medaka embryos. C Heatmap showing histone modification enrichment (ChIP-seq) around H3K27ac proximal peaks in normal medaka embryos. D Line plots indicate average enrichment of each histone modification around H3K27ac proximal peaks in normal medaka embryos. E Quantification of (C, D). Boxplots indicate average of histone modification enrichment in H3K27ac proximal peaks in normal medaka embryos. Two-sided Wilcoxon rank-sum test. Box plots indicate median (middle line), 25th, 75th percentile (box) and 5th and 95th percentile (whiskers), and outliers are not shown. n = 703, 398 for CBP/P300-dependent, CBP/P300-independent, respectively. F Schematic showing HATs and histone acetylations. G Heatmap showing expression levels (RNA-seq)⁷⁵ of genes encoding HATs in medaka. H Heatmap showing enrichment of transcription factor motifs that were specifically enriched (log2 fold change of p value > 1, log2 fold change of enrichment >0.1) in CBP/P300-dependent genes or -independent genes and the expression level of genes encoding the transcription factor in medaka embryos.