Fig. 4: Whole cell DNA-PAINT imaging within large field-of-views on an SDC-OPR microscope. | Nature Communications

Fig. 4: Whole cell DNA-PAINT imaging within large field-of-views on an SDC-OPR microscope.

From: Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment

Fig. 4

a Top: SDC-OPR DNA-PAINT image of microtubules in HeLa cells across a large FOV of 211 × 211 µm2. Cells were stained with DNA-labeled anti-alpha-tubulin antibodies, as described in the Methods section. Bottom: Distribution of localization precision (σSMLM) measured at different distances (in µm) from the center of the FOV, with each color representing a different distance. b SDC-OPR DNA-PAINT images of the microtubules at different penetration depths (0, 4, and 8 µm) within HeLa cells. A cartoon in the upper left corner provides a schematic representation of each penetration depth relative to the overall cell volume. c Distribution of σSMLM for each penetration depth for microtubules sample at 4× magnification, with each color representing a different penetration depth. d Left: Zoomed-in images of highlighted small regions from each penetration depth indicated in (b). Right: Position distribution for the highlighted regions in the lefthand panels, quantifying the distances between microtubule walls in nanometers. Microtubule imaging was repeated in three independent experiments. Source data of histograms are provided as a Source Data file. Schemes of (a, b) were created with BioRender.com.

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