Fig. 2: Frequency of innate cells in BAL (upper panels) and whole blood (lower panels) post-BCG infection. | Nature Communications

Fig. 2: Frequency of innate cells in BAL (upper panels) and whole blood (lower panels) post-BCG infection.

From: Early mucosal responses following a randomised controlled human inhaled infection with attenuated Mycobacterium bovis BCG

Fig. 2

BAL post-BCG or saline inhalation, or blood post-BCG inhalation. Stained with Panel 1. A Eosinophils were defined as live singlets, Lin- (CD19, CD56, CD3), CD16- Siglec8+ and shown as % CD3- cells. BAL D7 control v BCG p = 0.03, BCG D2 v D7 p = 0.0007; after correction BCG D2 v D7 p = 0.01. Blood baseline to D2 p = 0.03, D56 p = 0.045. B Neutrophils were defined as live singlets, Lin- (CD19, CD56, CD3), CD16+ , CD66b+ . Shown as % CD3- cells. BAL D2 control v BCG p = 0.007, BCG D2 v D7 p = 0.009. Blood baseline to D7 p = 0.03, D14 p = 0.07, D28 p = 0.003, D56 p = 0.003. After Dunns correction D7 p = 0.02, D28 p = 0.03, D56 p = 0.02. C Antigen presenting cells (APCs) refer to macrophages, monocytes and dendritic cells and were defined as live singlets, CD19-CD3-CD56-CD66b- CD45+ and shown as % leucocytes, defined as live singlets, CD19-. BAL D7 control v BCG p = 0.003, BCG D2 v D7 p = 0.0007; after correction BCG D2 v D7 p = 0.002. Blood baseline to D14 p = 0.03, D28 p = 0.006. After Dunns correction D28 p = 0.01. D Natural Killer (NK) cells defined as CD19-, CD3-, CD56+ to identify the total NK cell population as % CD3- cells; or E CD16+ NK cells as % total NK cell population. BAL NK cells BCG D2 v D7 p < 0.0001, after correction p = 0.0009. BAL CD16+ NK cells D7 control v BCG p = 0.007, BCG D2 v D7 p = 0.02. After correction D7 control v BCG p = 0.01. Blood baseline to D2 p = 0.04. Differences in cell frequency between BAL groups were calculated using a two-sided Mann-Whitney and corrected with Dunns; and in the blood, differences between baseline samples and corresponding timepoints following BCG inhalation were calculated using a paired two-sided Wilcoxon signed rank test against baseline only and corrected with Dunns. Statistically significant differences are presented in the figure, red asterix indicates significant differences after Dunn’s correction. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. See Supplementary Note 2 and source data for sample sizes per group. Source data are provided as a Source Data file. BAL: Black dots indicate BCG, grey squares saline control. Blood: Solid dots indicate Group 1 (D2 bronchoscopy) volunteers and circles represent Group 2 (D7 bronchoscopy) volunteers. Box and whisker plots show median, IQR and min/max.

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