Fig. 1: Elevated NAT10 expression is associated with decreased survival and reduced infiltration of immune cells in cancer. | Nature Communications

Fig. 1: Elevated NAT10 expression is associated with decreased survival and reduced infiltration of immune cells in cancer.

From: Inhibition of tumor-intrinsic NAT10 enhances antitumor immunity by triggering type I interferon response via MYC/CDK2/DNMT1 pathway

Fig. 1

A The expression of NAT10 was assessed in different stages of LUAD using the GEPIA website (http://gepia.cancer-pku.cn/). B Kaplan-Meier survival curve comparing the high- and low-NAT10 expression groups (optimal cut-off) in the TCGA-LUAD cohort. C Kaplan-Meier survival curve comparing the high- and low-NAT10 expression groups in 37 patients with lung cancer. NAT10 expression was quantified using immunohistochemistry and Image Pro Plus. Statistical significance was determined using the log-rank test. D ROC curves for survival prediction with corresponding AUC values. The area under the curve (AUC) was calculated, and the statistical significance of AUC comparison between groups was determined using the DeLong test. E, F Tumor weight and growth curves for C57BL/6 N mice inoculated with TC1 (E) or MCA205 tumor cells (F). 2 × 106 WT cells were subcutaneously inoculated into the back of C57BL/6 N mice; n = 5 mice per group. Mice received Remodelin or saline via oral gavage for the first 5 days at a dose of 100 mg/kg. Tumor size was measured daily using calipers to generate growth curves. Tumor growth curves were analyzed by two-way ANOVA with the tumor size at the final day used for significance testing. From left to right, **P = 0.004; ***P < 0.001; **P = 0.0015; ***P < 0.001, respectively. G, H Tumor weight and growth curves for nude/nude mice inoculated with TC1 (G) or MCA205 tumor cells (H). 2 × 106 WT cells were subcutaneously inoculated into the back of C57BL/6 N mice; n = 5 mice per group. Mice received Remodelin or saline via oral gavage for the first 5 days at a dose of 100 mg/kg. I Analysis of immune cell infiltration using the CIBERSORT algorithm between high- and low-NAT10 expression groups in the TCGA-LUAD cohort. J Immunohistochemical analysis of NAT10 expression and CD8+ T cell infiltration in patient-derived lung cancer samples (n = 37). CD8+ T cell counts in the high- and low-NAT10 expression groups are presented on the right. The arrow indicates CD8+ T cells; Scale bar: 50 µm; *P = 0.0484. Unless specified otherwise, the data are presented as means ± SEM (error bar) and compared using the two-sided Student’s t test; ns, no significance; Source data are provided as a Source Data file.

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