Fig. 7: Inhibition of NAT10 enhances the response to ICIs.

A Schematic diagram showing combination therapy of C57BL/6 N mice with Remodelin and ICIs. B, C Tumor weight for C57BL/6 N mice (n = 5 mice per group) inoculated with TC1 (B) or MCA205 (C) tumor cells treated with Remodelin and/or anti-PD-1 antibodies. TC1 or MCA205 tumor cells were inoculated subcutaneously into C57BL/6 N mice. Mice received Remodelin or saline via oral gavage for the first 7 days at a dose of 100 mg/kg. On day 8, mice were treated with IgG control or anti-PD-1 antibodies. After sacrificing the mice, tumor tissues were excised and representative images are shown in the left panel. Statistical significance was determined using One-way ANOVA; from left to right, all *** indicate P < 0.001. D, E Representative immunofluorescence staining of CD8⁺ T cells in TC1 (D) and MCA205 (E) tumor tissues. Tumor tissues from C57BL/6 N mice (B) were subjected to immunostaining for CD8⁺ T cells (red) and nuclei (blue). CD8⁺ T cells were quantified by counting positive signals in 3 randomly selected fields (20×) per tumor section using Image J. Statistical analysis was conducted using One-way ANOVA, n = 15 biologically independent samples. Scale bar, 100 µm; from left to right, all *** indicate P < 0.001. F, G FACS analysis of the proportions of IFN-γ⁺CD8⁺ immune cell populations in TC1 (F) and MCA205 (G) tumor tissues. Tumor tissues from C57BL/6 N mice (B) were subjected to FACS analysis for IFN-γ⁺CD8⁺ immune cells (n = 5 mice per group). Statistical significance was determined using One-way ANOVA; from left to right, ** P = 0.0027; ** P = 0.0063; ***P < 0.001; *** P < 0.001. H, I ELISpot assay was conducted to measure IFN-γ secretion in TC1 (H) and MCA205 (I) tumor tissues after different treatments. The number of spots and the results were quantified (n = 5 mice per group). Statistical significance was determined using One-way ANOVA; from left to right, ** P = 0.0039; ***P < 0.001; ** P = 0.0011; *** P = 0.0002. Unless specified otherwise, the data are presented as means ± SEM (error bar). Source data are provided as a Source Data file.