Fig. 1: scRNA analysis reveals LAM accumulation in BAT of obese mouse models.

A Heatmap of BAT and WAT markers in bulk RNAseq data of HFD and db/db BAT. B Heatmap of thermogenic proteins in proteomics data of db/db (upper panel) and HFD (lower panel) BAT. C Linear models fitted on scores computed on GO terms Macrophage Activation pathway and Adaptive Thermogenesis for BAT db/db data (upper panel) and BAT HFD data (lower panel). P-value by two-side ANOVA test. D Schematic figure delineating the scRNAseq experimental design. E UMAP representing cell clusters identified by scRNAseq of SVFs (n = 14,715) isolated from BAT of wild type (WT), db/db and high fat diet (HFD) mice. Clustering identified 5 major clusters: Fibro-Adipose Progenitors (FAP), Adipose Stromal Cells (ASC), Endothelial Cell, T cells and Monocytes/Macrophages (MonoMacs). F Barplot representing the cell proportion across the 3 conditions. G UMAP of MonoMacs sub-clustering (n = 5578) identified by scRNAseq analysis. Clustering identified 4 major clusters: Monocytes, Perivascular-like Macrophages (PVM), Lipid-Associated Macrophages (LAM) and Proliferating Macrophages. (H) UMAP of BAT MonoMacs splitted by condition. I Bar plot of MonoMacs subcluster abundance across the 3 conditions. J Multiplex immunofluorescence stained for Adiponectin (orange), UCP1 (yellow), TREM2 (green) and CD9 (grey) in paraffin-embedded tissue section from low fat diet (LFD) and HFD BAT. Nuclei stained with DAPI (blue). Scale bar, 20 μm.