Fig. 2: FoamSpotteR identifies foamy-like macrophages in obese BAT. | Nature Communications

Fig. 2: FoamSpotteR identifies foamy-like macrophages in obese BAT.

From: MACanalyzeR scRNAseq analysis tool reveals PPARγHIGH/GDF15HIGH lipid-associated macrophages facilitate thermogenic expansion in BAT

Fig. 2

A Graphical representation of the FoamSpotteR module workflow. B UMAP of macrophages isolated from atheromatous plaque (GSE97310 and GSE116240). Clustering identified two major clusters: foam macrophages (fMAC+) and non-foam macrophages (fMAC-). C, D UMAP of BAT macrophages colored by FoamSpotteR result (C) and splitted by sample (D). Values above 0.5 of FoamDEX identify foamy-like macrophages. E–G Gene Set Enrichment for GO Biological Process of shared upregulated genes in bulk RNAseq of foam macrophages and FoamSpotteR predicted fMAC+ (E), uniquely upregulated in foam macrophages (F) and uniquely upregulated in fMAC+ predicted cells (G) (p-value < 0.05; log2FC > 1). P-value by BH test.

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