Fig. 5: PPARγ LysMCre mice show an increase of body and BAT weight and loss of thermogenic proteins.

A, B scRNAseq velocities projected onto the UMAP in db/db MonoMacs (A) and HFD MonoMacs (B). C HubGenes of the PPI network of HFD velocity genes. D Expression of Pparg (upper panel) and PPAR signaling pathway (lower panel) splitted by conditions. Box plots show the median as center line, 25% and 75% percentiles as box limits and whiskers extending to the largest and smallest values. E Body weight analysis. Body weight time course during 10 weeks of LFD and HFD of WT and PPARγ LysMCre mice (left panel) and barplot reporting body weight after 10 weeks (right panel). F Barplot reporting BAT weight after 10 weeks of diet. G Heatmap of thermogenesis-related proteins in proteomics data of WT and PPARγ LysMCre mice in LFD and HFD (left panel) and barplot of UCP1 protein quantification (right panel). H H&E staining of BAT slices of HFD WT and HFD PPARγ LysMCre mice. Scale bar 250 μm. I Glucosemia after 10 weeks of LFD and HFD in WT and PPARγ LysMCre mice. J Blood cholesterol levels after 10 weeks of LFD and HFD in WT and PPARγ LysMCre mice. E–G, I, J Data are reported as mean ± SD. P-value by one-side ANOVA test. (n = 4 mice/group) (K) Representative immunoblot of UCP1 from BAT extracted from mice exposed to cold and transplanted with LFD (Mac LFD) and HFD (Mac HFD) BAT macrophages (left panel) and densitometry analysis (right panel). Tubulin staining was used as loading control (n = 3 mice/group). L Thermogenesis and lipid metabolism genes relative expression in BAT of mice exposed to cold and transplanted with LFD and HFD BAT macrophages. Data are reported as mean ± SD. P-value by unpaired student’s t test. (n = 3 mice/group). Source data are provided in the Source Data file.