Fig. 6: XL-3158 inhibits cGAS phase separation in cells.
From: De novo design of protein condensation inhibitors by targeting an allosteric site of cGAS
a Left: representative live-cell images of cGAS-DNA puncta formation in L929 cells stably expressing Tet-on EGFP-h-cGASFL, transfected with Cy5-labeled ISD100 (DNA), and treated with indicated compound (20 μM), n = 30. The first column shows full-field views with scale bars representing 20 μm. The other columns are enlarged views of the areas marked by white boxes in the first column. Green, EGFP-h-cGASFL; magenta, Cy5-labeled DNA. Scale bars, 10 μm. Right: quantified fluorescence intensities of different channels along a line perpendicular to one of the arrows in (left). The y-axis and x-axis represent the fluorescence intensity and the distance along the line, respectively. b Violin plot representation of numbers of h-cGASFL-DNA puncta counted from (a). Data are presented as mean ± SD from three independent experiments, with each experiment analyzing 10 different images. Statistical significance was determined by an unpaired two-tailed t test. ***p < 0.005. c FRAP of h-cGASFL-DNA puncta in cells. The puncta were formed as described in (a). Data represent the mean ± SD of five independent experiments. Scale bar, 2 μm. d Time-lapse micrographs of h-cGASFL-DNA merging puncta formed as described in (a). Data are representative of at least 3 merging puncta. Scale bar, 0.5 μm. Source data is available with this manuscript as a Source Data file.
