Fig. 8: XL-3158 attenuates cerulein-induced acute pancreatitis (AP).
From: De novo design of protein condensation inhibitors by targeting an allosteric site of cGAS
a Schematic illustration of the experimental protocol. Mice were intraperitoneally injected with cerulein (50 μg/kg) or saline (Vehicle) at 1-h intervals for a total of 7 injections. XL-3158 (25 mg/kg) or vehicle was administered by oral gavage at 0 h and 5 h after the first cerulein injection. Mice were euthanized 12 h after the last cerulein injection. b Pancreas-to-body weight ratio of mice from vehicle (saline), cerulein, and cerulein + XL-3158 groups. c Histopathological scores based on five parameters: edema, inflammatory infiltration, fat necrosis, parenchymal necrosis, and hemorrhages from different groups. d Representative H&E staining of pancreatic sections from different groups. Black, red, and green arrows indicate edema, inflammatory infiltration, and parenchymal necrosis, respectively. Scale bar = 200 µm. Relative serum amylase (e) and lipase (f) activity in different groups. Serum levels of TNF-α (g) and IL-6 (h) in different groups of mice were measured by ELISA. All quantitative data (b, c, e–h) are shown as scatter plots with horizontal lines indicating mean ± SD. For b, c, e, f: n = 10 biological replicates (individual mice) for vehicle and XL-3158 groups; n = 9 for cerulein group; g, h: n = 6 biological replicates (individual mice per group). Statistical significance determined by an unpaired two-tailed t test. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. Source data is available with this manuscript as a Source Data file.
