Fig. 4: Colonization of rabbit embryos by iPSCs overexpressing KLF2, ERAS and PRMT6.

a Confocal images of late-blastocyst-stage rabbit embryos (E5.0, 3DIV) following microinjection at the early morula-stage (E2.8) with B19_KF, KEPc#37_KF, KEPc#37_VAL, KEPc#44_KF, and KEPc#44_VAL cells. Scale bars: 50 µM. On the right, intensity profile plots were generated along a line drawn through the inner cell mass (ICM) of each embryo using Fiji. Fluorescence signals across channels (green: GFP; purple: SOX2) indicate co-localization of injected GFP⁺ cells with SOX2⁺ regions, suggesting integration into the pluripotent compartment. Images are representative of three independent experiments per cell line and condition. b Percentage of rabbit embryos containing GFP⁺ cells, based on three independent experiments for each cell line and condition. c Distribution of GFP⁺ embryos according to the number of GFP+ cells observed, corresponding to the embryos quantified in (b).