Fig. 6: Chimerism induced by KEP_VAL_CD75^high cells in rabbit fetuses.

a Experimental scheme for the isolation and analysis of CD75^high subpopulations from KEPi#28_VAL + S cells. b Flow cytometry plot showing sorting of the CD75^high subpopulation from KEP#28_VAL + S cells. c Confocal images of late-blastocyst-stage rabbit embryos (E5.0, 3DIV) after microinjection of KEPi#28_ CD75^high cells into morula-stage (E2.8) embryos. Images are representative of three independent experiments. Scale bars: 50 µm. d Percentage of embryos containing GFP⁺ cells for each condition. e Percentage of embryos containing more than 20 GFP⁺ cells per embryo. f GFP⁺ chimeric rabbit fetus was recovered at embryonic day 10.5 (E10.5) from two independent experiments. Scale bar: 200 µm. g Confocal images of transverse sections from an E10.5 chimeric fetus showing GFP⁺ cells labeled with anti-GFP antibody in multiple tissues: the neural tube (co-labeled with TUJ1 and SOX2), the heart (co-labeled with SMA), and the somites (co-labeled with PAX3). Images represent three technical replicates. Scale bars: 20 µm.