Fig. 4: Intracellular accumulation of AT8⁺ pTau and 4G8⁺ Aβ (intraAβ) in neuronal subtypes.

Proportions of pTau⁺ (a) and intraAβ⁺ (c) cells in each neuronal subtype cluster separately for CtrlCV (sections from n = 6 brains), CtrlTREM2 (n = 6), AlzCV (n = 18) and AlzTREM2 (n = 13) samples. Quantification was performed on three ROIs acquired from a single section of each sample and pooled together. Coloured boxes on the left highlight groups of neuronal types (excitatory, inhibitory and unclassified neurons) to which clusters were assigned to (“assigned neuronal type”). IMC images showing co-localisations of: RORB⁺ and AT8⁺ pTau in a AlzCV (Braak 5/6) tissue section (b); RORB⁺ neurons and 4G8⁺ Aβ in a CtrlCV (Braak 2) section (d); GAD1⁺ neurons and 4G8⁺ Aβ in a CtrlCV (Braak 0) section (e). Double-positive neurons (RORB⁺AT8⁺ in b; RORB⁺4G8⁺ in d; GAD1⁺4G8⁺ in e; yellow arrowheads) are found in the cortical layers shown in Fig. 3b, d, f. Source data are provided as a Source Data file. Scale bars in (b,d,e) represent 100 μm.