Fig. 6: Alcohol decreases TCA cycle metabolites, including lactate which depends on mTORC1. | Nature Communications

Fig. 6: Alcohol decreases TCA cycle metabolites, including lactate which depends on mTORC1.

From: Paradoxical mTORC1-Dependent microRNA-mediated Translation Repression in the Nucleus Accumbens of Male Mice Consuming Alcohol Attenuates Glycolysis

Fig. 6

A Aldolase A in the glycolysis pathway converts F1,6BP to G3P and DHAP. Lactate is the final product of glycolysis. B After 7 weeks of IA20%2BC (Supplementary Table 1) or water only, the NAc was dissected after 24 h of alcohol withdrawal and metabolite levels were measured. Data are presented as relative amount of individual metabolites in water vs. alcohol. Data are presented as individual data points and mean ± SEM. *p < 0.05, **p < 0.01, ns: non-significant. # metabolites that were not included in the panel. Water control: n = 6 mice, Alcohol withdrawal: n = 7 mice. Significance was determined using two-tailed Mann-Whitney test. Lactate U = 4, p = 0.0140; Citrate U = 6, p = 0.035; a-Ketogluturate U = 6, p = 0.035; Malate U = 3, p = 0.0082. C Mice underwent 7 weeks of IA20%2BC (Supplementary Table 1) or water only. Three hours before the end of the last alcohol withdrawal period, mice were systemically injected with 20 mg/kg rapamycin or vehicle. The NAc was dissected after 3 h, and lactate level was measured using a colorimetric assay. Data are presented as individual data points and mean ± SEM. Each data point represents an average of 3 technical replicates. **p < 0.01, ***p < 0.001. n = 6 mice per group. Significance was determined using One-way ANOVA followed by Tukey’s multiple comparisons test. F(2, 15) = 13.48, p = 0.0004. Water/vehicle vs alcohol/vehicle, p = 0.0005; alcohol/vehicle vs alcohol/rapamycin, p = 0.004. D Alcohol reduces TCA metabolites in the NAc and lactate which depends on mTORC1. Source data are provided as a Source Data file.

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