Fig. 7: Overexpression of miR-34a in NAc D1 neurons increases alcohol consumption but does not affect locomotion or sucrose intake. | Nature Communications

Fig. 7: Overexpression of miR-34a in NAc D1 neurons increases alcohol consumption but does not affect locomotion or sucrose intake.

From: Paradoxical mTORC1-Dependent microRNA-mediated Translation Repression in the Nucleus Accumbens of Male Mice Consuming Alcohol Attenuates Glycolysis

Fig. 7

A Experimental timeline. Mice received a bilateral infusion of AAV2-Flex-miR-34a or AAV2-Flex-control in the NAc shell of D1-Cre mice. Three weeks after surgery, mice underwent 2 weeks of IA20%2BC followed by 2 weeks of water only and 2 weeks of IA1%Sucrose2BC. Mice were then subjected to the open field test. B The NAc of D1-Cre mice was infected with AAV2-Flex-control or AAV2-Flex-miR-34a-GFP. Three weeks later, mice underwent IA20%2BC, and alcohol consumption was measured daily for 6 sessions. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Control group: n = 10 mice, D1-specific miR-34a OE group: n = 9 mice. Significance was determined using Two-way ANOVA Mixed-effects followed by Sidak’s multiple comparisons test. miR34a-5p overexpression x Time: F (5, 84) = 1.036, p = 0.4017; miR34a-5p overexpression: F (1, 17) = 16.46, p = 0.0008; Time: F (5, 84) = 3.698, p = 0.0045. Session 1, p = 0.0037; Session 2, p < 0.0001; Session 3, p = 0.0578; Session 4, p = 0.0089; Session 5, p = 0.0277; Session 6, p = 0.061. C The NAc of D1-Cre mice was infected with AAV2-Flex-control or AAV2-Flex-miR-34a. Mice were placed in an open field and mice movement was recorded for 20 min. Locomotion is depicted in 1-minute bins. Data are presented as mean ± SEM. ns: non-significant. Control group: n = 10 mice, D1-specific miR-34a OE group: n = 9 mice. Significance was determined using Two-way ANOVA RM. miR34a-5p overexpression x Time: F(5, 85) = 0.3717, p = 0.8667; miR34a-5p overexpression: F(5, 85) = 0.3105, p = 0.5846; Time: F(5, 85) = 1.623, p = 0.1627. D AAV2-Flex-control or AAV2-Flex-miR-34a-GFP infected mice underwent 2 weeks of IA1%Sucrose2BC. Data are presented as mean ± SEM. ns: non-significant. Control group: n = 10 mice, D1-specific miR-34a OE group: n = 9 mice. Significance was determined using Two-way ANOVA RM. miR34a-5p overexpression x Time: F(19, 323) = 2.614, p = 0.0003; miR34a-5p overexpression: F(1, 17) = 1.148, p = 0.2990; Time: F(17, 323) = 10.58, p < 0.0001. Source data are provided as a Source Data file.

Back to article page