Fig. 7: Late type 1a and type 1b CPECs with prenatal lineage summary. | Nature Communications

Fig. 7: Late type 1a and type 1b CPECs with prenatal lineage summary.

From: Sequential emergence and contraction of epithelial subtypes in the prenatal human choroid plexus revealed by a stem cell model

Fig. 7

A Venn diagram of significantly-enriched KEGG pathways (p < 0.05 with Bonferroni correction) based on top-300 DEGs of C1a and C1b cells at 75 div. C1b cells (purple) are distinctively enriched for catabolic processes, while C1a cells (pink) share many metabolic and energy-associated pathways enriched in 31-div C1 cells (Fig. 6A). See Supplementary Fig. 9A and Supplementary Data 1 for directed GSEA analyses of anabolic-secretory and catabolic-absorptive pathways. B Enriched GSEA terms for C1a (pink) and C1b cells (purple) using filtered genes (see Methods) expressed by >25% of cells. C Annotated GSEA theme plot illustrating C1a and C1b pathway enrichments. D Pearson correlations among (red-yellow) or between (green-violet) C1a and C1b marker gene pairs. Note the increase in some correlations at 55 div before their strong anti-correlations by 75 div. E 75 div heatmap of C1a and C1b marker genes used in panels D–K and Supplementary Fig. 9C–K. Color scale of Z-score of DEGs. F–H C1a-C1b specification in vitro (CLDN5-SC5D ICC maximal projections with magnified insets and rank-order heatmaps with Spearman correlations; 61 and 79 div). Unlike CLDN5-SC5D coexpression at 61 div (Supplementary Fig. 9F), discrete regions of CLDN5hi-SC5Dlo (C1a) and SC5Dhi-CLDN5lo (C1b) cells are evident by 79 div (F, G). A trend towards positive CLDN5-SC5D correlation at 61 div (p = 0.2n.s.; n = 150 cells) becomes significantly negative by 79 div (p < 0.0001****; n = 584 cells) (H). I–K C1a-C1b specification in vivo (CLDN5-SC5D IHC maximal projections with magnified insets and rank-order heatmaps with Spearman correlations; 23 and 42 pcw). Similar to in vitro, discrete regions of CLDN5hi-SC5Dlo (C1a) and SC5Dhi-CLDN5lo (C1b) cells are evident by 42 pcw. At 23 pcw, CLDN5-SC5D correlation is significantly positive (p < 0.001***; n = 323 cells) before becoming significantly negative by 42 pcw (p < 0.001***; n = 812 cells). Color scale of fluorescence levels for CLDN5 and SC5D. L Summary of the CPEC lineage tree with in vitro and in vivo timepoints. Source data provided as a Source Data file. See Statistics and Reproducibility section of Methods for additional information.

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