Fig. 2: Reporter assay of split ON switch system. | Nature Communications

Fig. 2: Reporter assay of split ON switch system.

From: Split RNA switch orchestrates pre- and post-translational control to enable cell type-specific gene expression

Fig. 2

a Schematic illustration of the strategy to improve the ON/OFF ratio of ON switch systems. Introducing a “leak-canceller”, an OFF switch coding an inactive C-terminal fragment, together with the split ON switch, enables the suppression of leaky protein activity in miRNA-cells and enhances the ON/OFF ratio. b Relative hmAG1 intensity (hmAG1/iRFP670) of HEK293FT cells treated with miR-21-5p or negative control (NC) mimics. a.u., arbitrary units. Error bars represent means ± SD (n = 3), and data of each biological replicate are shown as a point. Statistical analysis by two-sided Welch’s ttest, **P < 0.01. Each P-value is listed in Supplementary Table 4. Source data are provided as a Source Data file. c Representative microscopic images of HEK293FT cells transfected in the same conditions as shown in (b). Green fluorescence (left) and bright-field (right) images are shown for each condition. Scale bar, 200 µm. d Representative 2D flow cytometry plots. The horizontal axis shows the fluorescence intensity of iRFP670 (reference), and the vertical axis shows the fluorescence intensity of hmAG1 (reporter).

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